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bamhi hindiii restriction sites  (New England Biolabs)


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    Structured Review

    New England Biolabs bamhi hindiii restriction sites
    Bamhi Hindiii Restriction Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1827 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bamhi+hindiii/BamHI-HF/pm41932493-240-10-19
    Average 99 stars, based on 1827 article reviews
    bamhi hindiii restriction sites - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Molecular characterization of Rft1, an ER membrane protein associated with congenital disorder of glycosylation RFT1-CDG
    Article Snippet: .. Plasmid construction was as follows: EcAKM135 (hRft1-3xFLAG), EcAKM141 (hRft1 R67C -3xFLAG), and EcAKM187 (p416-P GPD -hRFT1-3xFLAG): hRft1-3xFLAG and hRft1 R67C -3xFLAG fragments were PCR amplified from plasmids AP63 and AP65, respectively, and inserted into BamHI-HindIII-digested p413 -P GPD or p416 -P GPD vector using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, E5520S). .. EcAKM147 (P GPD -ALFA-mNG-hRft1-3xFLAG): the mNG fragment was amplified from pFA6a-2xmNeonGreen-kanMX and inserted into EcAKM135 using the NEBuilder HiFi DNA Assembly kit to construct EcAKM136 (mNG-hRft1-3xFLAG).

    Article Title: Cobalt mitigates zinc-starvation effects in Pseudomonas aeruginosa
    Article Snippet: .. The EcoRI/BamHI and BamHI/HindIII (New England Biolabs) digested 5' and 3' fragments and the gentamicin resistance cassette were cloned in plasmid pEX18Tc, and the resulting plasmid was mobilized to PA14 wild-type by tri-parental mating as already described (D’Orazio et al. ). ..

    Article Title: Animal feed compositions and uses thereof
    Article Snippet: The fragment was received from GeneArt in kanamycin resistant vector pMK and the plasmid was dissolved DNA in 50 uls in 10 mM Tris, pH7.5. .. 20 uls of the mixture with BamHI-HindIII according to the protocol below: Plasmid 20 μl Cut smart buffer: 10x #B7204S 4.0 μl Milli-Q H2O: 14.0 μl Hind III HF: NEB #R3104S 1 μl BamH HF: NEB #R3136S 1 μl Total volume 40 μl The reaction was incubated for 3 hours at 37° C. The restriction digest was purified with the GFX PCR DNA and Gel Band purification Kit (Cat.no 28-9034-71) from GE Healthcare and eluted in 40 uls 10 mM Tris, pH 7.5. .. The purified restriction digest was then used in a simple ligation with BamHI-HindIII restricted pDau109 (example 1) according to the T4 DNA ligase manufacturer's instructions (New England Biolabs., neb.com).

    Article Title: Molecular characterization of Rft1, an ER membrane protein associated with congenital disorder of glycosylation RFT1-CDG.
    Article Snippet: .. Plasmid construction was as follows: EcAKM135 (hRft1-3xFLAG), EcAKM141 (hRft1R67C-3xFLAG) and EcAKM187 (p416-PGPD-hRFT13xFLAG): hRft1-3xFLAG and hRft1R67C-3xFLAG fragments were PCR amplified from plasmids AP63 and AP65, respectively, and inserted into BamHI-HindIII-digested p413-PGPD or p416-PGPD vector using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, E5520S). .. EcAKM147 (PGPD-ALFA-mNG-hRft1-3xFLAG): the mNeonGreen fragment was amplified from pFA6a-2xmNeonGreen-kanMX and inserted into EcAKM135 using the NEBuilder HiFi DNA Assembly kit to construct EcAKM136 (mNG-hRft1-3xFLAG).

    Article Title: Cobalt mitigates zinc-starvation effects in Pseudomonas aeruginosa.
    Article Snippet: .. The EcoRI/BamHI and BamHI/HindIII (New England Biolabs) digested 5’ and 3’ fragments and the gentamicin resistance cassette were cloned in plasmid pEX18Tc, and the resulting plasmid was mobilized to PA14 wild-type by tri-parental mating as already described (D’Orazio et al. 2015). ..

    Polymerase Chain Reaction:

    Article Title: Molecular characterization of Rft1, an ER membrane protein associated with congenital disorder of glycosylation RFT1-CDG
    Article Snippet: .. Plasmid construction was as follows: EcAKM135 (hRft1-3xFLAG), EcAKM141 (hRft1 R67C -3xFLAG), and EcAKM187 (p416-P GPD -hRFT1-3xFLAG): hRft1-3xFLAG and hRft1 R67C -3xFLAG fragments were PCR amplified from plasmids AP63 and AP65, respectively, and inserted into BamHI-HindIII-digested p413 -P GPD or p416 -P GPD vector using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, E5520S). .. EcAKM147 (P GPD -ALFA-mNG-hRft1-3xFLAG): the mNG fragment was amplified from pFA6a-2xmNeonGreen-kanMX and inserted into EcAKM135 using the NEBuilder HiFi DNA Assembly kit to construct EcAKM136 (mNG-hRft1-3xFLAG).

    Article Title: Animal feed compositions and uses thereof
    Article Snippet: The fragment was received from GeneArt in kanamycin resistant vector pMK and the plasmid was dissolved DNA in 50 uls in 10 mM Tris, pH7.5. .. 20 uls of the mixture with BamHI-HindIII according to the protocol below: Plasmid 20 μl Cut smart buffer: 10x #B7204S 4.0 μl Milli-Q H2O: 14.0 μl Hind III HF: NEB #R3104S 1 μl BamH HF: NEB #R3136S 1 μl Total volume 40 μl The reaction was incubated for 3 hours at 37° C. The restriction digest was purified with the GFX PCR DNA and Gel Band purification Kit (Cat.no 28-9034-71) from GE Healthcare and eluted in 40 uls 10 mM Tris, pH 7.5. .. The purified restriction digest was then used in a simple ligation with BamHI-HindIII restricted pDau109 (example 1) according to the T4 DNA ligase manufacturer's instructions (New England Biolabs., neb.com).

    Article Title: Bispecific targeting system based on DARPins-modified light-chain ferritin for cancer chemotherapy.
    Article Snippet: Cancer continues to be one of the leading causes of death worldwide.. Due to genome instability, solid tumors are characterized by high heterogeneity of tumor-associated antigen expression, which makes a great challenge for targeted tumor therapy.. Targeting two molecular receptors overexpressed on tumor cells is a way to overcome this problem and improve therapeutic efficacy.

    Article Title: Molecular characterization of Rft1, an ER membrane protein associated with congenital disorder of glycosylation RFT1-CDG.
    Article Snippet: .. Plasmid construction was as follows: EcAKM135 (hRft1-3xFLAG), EcAKM141 (hRft1R67C-3xFLAG) and EcAKM187 (p416-PGPD-hRFT13xFLAG): hRft1-3xFLAG and hRft1R67C-3xFLAG fragments were PCR amplified from plasmids AP63 and AP65, respectively, and inserted into BamHI-HindIII-digested p413-PGPD or p416-PGPD vector using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, E5520S). .. EcAKM147 (PGPD-ALFA-mNG-hRft1-3xFLAG): the mNeonGreen fragment was amplified from pFA6a-2xmNeonGreen-kanMX and inserted into EcAKM135 using the NEBuilder HiFi DNA Assembly kit to construct EcAKM136 (mNG-hRft1-3xFLAG).

    Article Title: G-quadruplex forming regions in GCK and TM6SF2 are targets for differential DNA methylation in metabolic disease and hepatocellular carcinoma patients
    Article Snippet: To verify the regulatory role of the DMPs identified in GCK exon 7 and TM6SF2 intron 2-exon 3 boundary, the regions were cloned into pCpG-free vectors (Invivogen, Belgium). .. The PCR primers contained restriction enzyme (RE) cutting sites for NsiI (NEB, Austria) and BamHI / HindIII (NEB, Austria) at the 5 ́ends of the forward and reverse primers, respectively. .. 500 ng of each, PCR amplicon and plasmid, were double-digested at 37°C for 20 min and cleaned up with a DNA Clean-up and Concentration System (Promega, Austria).

    Amplification:

    Article Title: Molecular characterization of Rft1, an ER membrane protein associated with congenital disorder of glycosylation RFT1-CDG
    Article Snippet: .. Plasmid construction was as follows: EcAKM135 (hRft1-3xFLAG), EcAKM141 (hRft1 R67C -3xFLAG), and EcAKM187 (p416-P GPD -hRFT1-3xFLAG): hRft1-3xFLAG and hRft1 R67C -3xFLAG fragments were PCR amplified from plasmids AP63 and AP65, respectively, and inserted into BamHI-HindIII-digested p413 -P GPD or p416 -P GPD vector using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, E5520S). .. EcAKM147 (P GPD -ALFA-mNG-hRft1-3xFLAG): the mNG fragment was amplified from pFA6a-2xmNeonGreen-kanMX and inserted into EcAKM135 using the NEBuilder HiFi DNA Assembly kit to construct EcAKM136 (mNG-hRft1-3xFLAG).

    Article Title: Molecular characterization of Rft1, an ER membrane protein associated with congenital disorder of glycosylation RFT1-CDG.
    Article Snippet: .. Plasmid construction was as follows: EcAKM135 (hRft1-3xFLAG), EcAKM141 (hRft1R67C-3xFLAG) and EcAKM187 (p416-PGPD-hRFT13xFLAG): hRft1-3xFLAG and hRft1R67C-3xFLAG fragments were PCR amplified from plasmids AP63 and AP65, respectively, and inserted into BamHI-HindIII-digested p413-PGPD or p416-PGPD vector using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, E5520S). .. EcAKM147 (PGPD-ALFA-mNG-hRft1-3xFLAG): the mNeonGreen fragment was amplified from pFA6a-2xmNeonGreen-kanMX and inserted into EcAKM135 using the NEBuilder HiFi DNA Assembly kit to construct EcAKM136 (mNG-hRft1-3xFLAG).

    Clone Assay:

    Article Title: Cobalt mitigates zinc-starvation effects in Pseudomonas aeruginosa
    Article Snippet: .. The EcoRI/BamHI and BamHI/HindIII (New England Biolabs) digested 5' and 3' fragments and the gentamicin resistance cassette were cloned in plasmid pEX18Tc, and the resulting plasmid was mobilized to PA14 wild-type by tri-parental mating as already described (D’Orazio et al. ). ..

    Article Title: Bispecific targeting system based on DARPins-modified light-chain ferritin for cancer chemotherapy.
    Article Snippet: Cancer continues to be one of the leading causes of death worldwide.. Due to genome instability, solid tumors are characterized by high heterogeneity of tumor-associated antigen expression, which makes a great challenge for targeted tumor therapy.. Targeting two molecular receptors overexpressed on tumor cells is a way to overcome this problem and improve therapeutic efficacy.

    Article Title: Cobalt mitigates zinc-starvation effects in Pseudomonas aeruginosa.
    Article Snippet: .. The EcoRI/BamHI and BamHI/HindIII (New England Biolabs) digested 5’ and 3’ fragments and the gentamicin resistance cassette were cloned in plasmid pEX18Tc, and the resulting plasmid was mobilized to PA14 wild-type by tri-parental mating as already described (D’Orazio et al. 2015). ..

    Incubation:

    Article Title: Animal feed compositions and uses thereof
    Article Snippet: The fragment was received from GeneArt in kanamycin resistant vector pMK and the plasmid was dissolved DNA in 50 uls in 10 mM Tris, pH7.5. .. 20 uls of the mixture with BamHI-HindIII according to the protocol below: Plasmid 20 μl Cut smart buffer: 10x #B7204S 4.0 μl Milli-Q H2O: 14.0 μl Hind III HF: NEB #R3104S 1 μl BamH HF: NEB #R3136S 1 μl Total volume 40 μl The reaction was incubated for 3 hours at 37° C. The restriction digest was purified with the GFX PCR DNA and Gel Band purification Kit (Cat.no 28-9034-71) from GE Healthcare and eluted in 40 uls 10 mM Tris, pH 7.5. .. The purified restriction digest was then used in a simple ligation with BamHI-HindIII restricted pDau109 (example 1) according to the T4 DNA ligase manufacturer's instructions (New England Biolabs., neb.com).

    Purification:

    Article Title: Animal feed compositions and uses thereof
    Article Snippet: The fragment was received from GeneArt in kanamycin resistant vector pMK and the plasmid was dissolved DNA in 50 uls in 10 mM Tris, pH7.5. .. 20 uls of the mixture with BamHI-HindIII according to the protocol below: Plasmid 20 μl Cut smart buffer: 10x #B7204S 4.0 μl Milli-Q H2O: 14.0 μl Hind III HF: NEB #R3104S 1 μl BamH HF: NEB #R3136S 1 μl Total volume 40 μl The reaction was incubated for 3 hours at 37° C. The restriction digest was purified with the GFX PCR DNA and Gel Band purification Kit (Cat.no 28-9034-71) from GE Healthcare and eluted in 40 uls 10 mM Tris, pH 7.5. .. The purified restriction digest was then used in a simple ligation with BamHI-HindIII restricted pDau109 (example 1) according to the T4 DNA ligase manufacturer's instructions (New England Biolabs., neb.com).

    other:

    Article Title: G-quadruplex forming regions in GCK and TM6SF2 are targets for differential DNA methylation in metabolic disease and hepatocellular carcinoma patients.
    Article Snippet: Briefly, DNA was isolated from HepG2 cells and the regions were amplified in 50 μL reaction volumes containing 1 × GC buffer, 200 μM dNTPs, 0.5 μM each forward and reverse primer, and 0.5 U Phusion HSII polymerase (Thermo Scientific, Austria).



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